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This content is taken from the University of Birmingham's online course, Metabolomics: Understanding Metabolism in the 21st Century. Join the course to learn more.

Welcome to Week 3

In this penultimate week of the course we will discuss experimental design, sample preparation and introduce the analytical techniques applied to study the metabolome.

The design of experiments is important in any scientific experiment. In metabolomics it is very important to ensure confounding factors are not introduced into the studying and that all sources of variance are controlled with the exception of the one under investigation.

A vast range of samples may be used in metabolomics studies, we will look at the different sample types that are commonly analysed and discuss the preparation methods that may be applied to extract the different classes of chemicals.

A number of analytical approaches may be applied to measure the metabolome. In this course we focus on the application of mass spectrometry and liquid chromatography coupled to mass spectrometry to analyse the metabolome, considering the advantages and applications of both approaches and illustrate how we are developing these approaches at the University of Birmingham.

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This article is from the free online course:

Metabolomics: Understanding Metabolism in the 21st Century

University of Birmingham