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Week 2 summary

Summary PCR, RT-PCR, digital PCR, LAMP
© Universiti Malaya

In week 2, you have learned a few molecular methods for the diagnosis of parasitic infection. These methods offer a sensitive and specific approach to the detection of parasites. In situ, hybridization allows for the detection of specific nucleic acid sequences within an infected tissue sample. Conventional PCR is a widely used technique for detecting parasitic DNA in biological samples. Nested PCR and multiplex PCR offer increased specificity and sensitivity. Real-time PCR allows for the quantification of the parasitic load in a sample.

LAMP is an alternative to PCR, which does not require thermal cycling and can be performed in a single step. RAPD, AFLP, and RFLP are used mainly for strain identification and genetic variability analysis. The combination of serological and molecular techniques enhances the diagnostic accuracy of parasitic infections. Immunocapture-PCR improves the specificity and sensitivity of PCR by capturing the parasites from a complex mixture. Molecular diagnostic methods have revolutionized the detection and monitoring of parasitic infections, allowing for better disease management and control. Below are key points to take note of for each method:

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© Universiti Malaya
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Advanced Research Techniques in Parasitology

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