FRB Against SHRSP

So we evaluated this fermented rice bran and non-fermented rice bran using similar system and using SHRSP. We prepared 5% of fermented rice bran or non-fermented rice bran for the experimental diet and fed to 10 weeks old SHRSP for 4 weeks. You can see blood pressure was significantly reduced in both fermented and non-fermented rice bran groups. Serum ACE, angiotensin-converting enzyme, inhibitory activity was significantly increased in fermented rice bran group. Angiotensin-converting enzyme is very important for blood pressure upregulating. So fermented rice bran and rice bran also inhibit inhibit the activity of this ACE enzyme. Unfortunately, in case of the blood pressure parameters,

there is no statistical difference between fermented and non-fermented rice bran. So, next we checked glucose metabolism during the feeding of experimental diet. We measured blood glucose and insulin levels. Fermented rice bran decreased non-fasting plasma glucose and insulin levels in SHRSP, And much effectively compared with non-fermented rice bran After three weeks administration, we had done OGTT (oral glucose tolerance test) and ITT (insulin tolerance test) experiments. Fermented rice bran supplementation significantly improved OGTT, glucose and insulin, and insulin tolerance test and HOMA-IR. This means fermentation process enhances glucose metabolism, improved the activity. And we checked the serum adiponectin level, because maybe you remember the previous experiment when we treated with Driselase fraction of the rice bran, enhanced the adiponectin level.

So we checked the serum adiponectin level in this experiment also. Serum adiponectin was significantly increased by fermented rice bran. And blood adiponectin level maybe contribute to the glucose metabolism and lipid metabolism in liver. So we checked AMPK level in liver. AMPK is one of the target molecules of adiponectin, described at the right panel Activated form of AMPK is actually phosphorylated one. And phosphorylated AMPK was significantly enhanced in fermented rice bran group

This is the summary of this result. Fermented rice bran significantly reduced blood pressure in SHRSP. but not statistically different from, compared with the non-fermented one. But fermented rice bran showed significantly higher ACE inhibitory activity in serum. And also, fermented rice bran increased serum adiponectin levels and phosphorylation of hepatic AMPK

which contributes to improve lipid metabolism and insulin resistance.