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Molecular Biology: How to Detect Pathogens in Aquaculture

Gain an introduction to pathogens as you learn how to use PCR to analyse DNA and identify infectious diseases.

Shrimps swimming in a pool of DNAs and molicues

Molecular Biology: How to Detect Pathogens in Aquaculture

  • 4 weeks

  • 2 hours per week

  • Digital certificate when eligible

  • Intermediate level

Find out more about how to join this course

Develop essential skills for disease prevention

Molecular biology techniques have become a well-established, accurate, and effective way to obtain pathogen detection results. This makes these techniques very powerful not only in aquatic pathogen detection but also other industries such as animal agriculture and in hospitals for human disease.

On this four-week course, you’ll learn how to perform different molecular biology techniques for effective aquatic pathogen detection.

Using white shrimp as your test subject, you’ll develop the skills to identify diseases, detect pathogens, and summarise your results in a report that can help with disease control.

Understand the steps of RNA extraction

You’ll start by examining the anatomy of a white shrimp to help you identify the signs of three common diseases. With this knowledge, you’ll then explore how to prevent these diseases.

Next, you’ll learn DNA and total RNA extraction techniques to help you begin your analysis of white shrimp tissue.

Learn the procedures of cDNA synthesis and polymerase chain reaction

Polymerase chain reaction (PCR) plays an important role in the early detection and prevention of infectious diseases in aquaculture.

On the course, you’ll explore different PCR techniques, such as cDNA synthesis, to ensure you can detect shrimp viruses and potential causes of shrimp diseases.

Perform pathogen detection using molecular biology techniques

Finally, you’ll learn practical skills to perform pathogen detection using the molecular biology techniques explained on this course.

By the end, you’ll know how to collect and dissect diseased shrimp from farms, identify clinical signs of diseased shrimp, and perform tissue collections.


  • Week 1

    Introduction of aquatic pathogen detection

    • Aquaculture pathogen detection and disease identification

      Identifying disease in farmed aquatic species is critical for food security and ecosystem health. Prof Lee will introduce aquaculture dieseases in this session.

  • Week 2

    Chromosomal DNA extraction from white shrimp tissues

    • Anatomy of white shrimp

      Prof Lee will introduce shrimp dissection and genomic DNA extraction for PCR analysis.

  • Week 3

    RNA extraction from white shrimp tissues

    • RNA extraction and cDNA synthesis

      In order to synthesize cDNA, what method is used to extract RNA and reverse transcription, PCR and finer temperature controling to synthesize cDNA.

  • Week 4

    Procedures of RNA extraction and cDNA synthesis

    • Exploring PCR Techniques

      Controlling the temperature and RNA reverse transcription to separate the DNA and the action of the primer, PCR can increase the DNA and reach a detectable Ct value. Use the Livak method and the Pfaffl method.

    • Real-time PCR primer design

      Introduces that in order to prevent false positives in PCR , adjust the way primers with DNA or RNA to ensure the correctness of results, and its application.

    • Assessment time and course feedback

      The course will end here. If you want to earn a Certificate of Achievement on this course, please take the test for a quick review. You are welcome to leave any feedback and rethink how to bring it forward.

When would you like to start?

Start straight away and join a global classroom of learners. If the course hasn’t started yet you’ll see the future date listed below.

  • Available now

Learning on this course

On every step of the course you can meet other learners, share your ideas and join in with active discussions in the comments.

What will you achieve?

By the end of the course, you‘ll be able to...

  • Explore anatomy of white shrimp
  • Interpret chromosomal DNA extraction from white shrimp tissues
  • Interpret the steps of RNA extraction from white shrimp tissues
  • Explain procedures of RNA Extraction and cDNA Synthesis
  • Explain steps for standard polymerase chain reaction (PCR)
  • Interpret steps for real-time quantitative polymerase chain reaction (qRT-PCR)

Who is the course for?

This course is designed for anyone interested in molecular biology and pathogen detection.

It will be most beneficial for students, biologists, or researchers who would like to learn the basics of tissue collection, nucleic acid extraction, and pathogen detection in white shrimp.

Who will you learn with?

Who developed the course?

Taipei Medical University

Taipei Medical University (TMU) has been a leader in Taiwan’s healthcare system for over half a century due to its research, teaching excellence, internationalization and outstanding medical service.

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Ways to learn

Choose the best way to learn for you!

Subscribe & save


For the first two months. Automatically renews

Develop skills to further your career

  • Access to this course
  • Access to 1,000+ courses
  • Learn at your own pace
  • Discuss your learning in comments
  • Digital certificate when you're eligible

Cancel for free anytime

Buy this course

$129/one-off payment

Fulfill your current learning need

  • Access to this course
  • Learn at your own pace
  • Discuss your learning in comments
  • Printed and digital certificate when you’re eligible

Limited access


Sample the course materials

  • Access expires 7 Jan 2024

Find out more about certificates, Unlimited or buying a course (Upgrades)

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