An introduction to pharmacogenomics
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St George's, University of London online course,
The Genomics Era: the Future of Genetics in Medicine
AbacavirAbacavir is a commonly-used and highly effective nucleoside reverse transcriptase inhibitor (NRTI) used in combination with other drugs to reduce viral load in HIV.Upwards of 5% of patients will experience severe hypersensitivity when taking it; a rash, diarrhoea, fatigue and flu-like symptoms. It was discovered that those patients were more likely to have a particular variant, HLA-B*5701 in the major histocompatibility complex (MHC).Clinical trials showed that screening patients for HLA-B*5701 before starting treatment with abacavir significantly reduced the number of patients suffering this immune-mediated hypersensitivity.Today, this screening test forms a routine part of HIV medicine.1
Thiopurine drugs e.g. azathioprineThe thiopurine drugs, such as azathioprine, are immunosuppressants used in the prevention of organ transplant rejection, as well as in acute lymphoblastic leukaemia and in a variety of inflammatory and autoimmune diseases, such as inflammatory bowel disease and rheumatoid arthritis.Azathioprine is converted into its active form by an enzyme called thiopurine S-methyltransferase (TPMT). Some variants of the gene encoding TPMT reduce or abolish its function and result in unconverted azathioprine building up in the bone marrow.Patients who have two variant low activity alleles (TPMT deficiency) will, with 100% certainty, develop life-threatening myelosuppression at conventional thiopurine dosages; drastic dose reduction (e.g. 10% of conventional dose) is required.Those who have one lower-activity allele (heterozygotes) will need a degree of dose-adjustment but not to the same degree. The testing is relatively cheap and prevents the 1 in 300 chance of life-threatening complications.Consequently, TPMT testing is recommended by the BNF (British National Formulary) prior to starting treatment with thiopurines and this has been adopted by a wide range of medical specialties in the UK.2
WarfarinIt is also worth mentioning the drug that has been the focus of the largest amount of pharmacogenomic work to date: warfarin, a vitamin K antagonist widely used to prevent blood clots in at-risk individuals.Use of the drug involves a careful monitoring process, since the therapeutic window – the level needed to prevent thrombosis and not cause bleeding – is very narrow. Warfarin works by blocking the enzyme vitamin K epoxide reductase, encoded for by a gene called VKORC1.There is promising data suggesting that variants in VKORC1 as well as the CYP2C9 gene, responsible for metabolizing the pharmacologically active S-warfarin isomer, confer an increased sensitivity to warfarin. However, the efficacy of such testing in clinical practice is unclear from randomised controlled trials.Consequently, groups such as the British Committee for Standards in Haematology and the American College of Chest Physicians, have so far recommended against routine testing.3, 4
Imatinib (Gleevec)Chronic myelogenous leukaemia (CML) is a slowly progressing cancer of the blood and bone marrow characterised by the production of too many white blood cells. 95% of patients with CML have a specific chromosomal rearrangement between chromosomes 9 and 22 known as the ‘Philadelphia chromosome’.This rearrangement causes two genes, ABL and BCR, to join together to make a fusion gene called BCR-ABL. Under normal circumstances, ABL produces a tyrosine kinase enzyme, that helps to control cell growth and division.The abnormal fusion gene, BCR-ABL, results in the tyrosine kinase activity being entirely unregulated. This results in white blood cells that divide quickly and continuously, leading to massive numbers of cells being produced.The drug imatinib, (trade name Glivec or Gleevec) targets the abnormal tyrosine kinase activity to block cell division. It induces complete remission in over 80% of patients with chronic-phase CML.5
Trastuzumab (Herceptin)HER2: HER2 is a protein that is expressed in high levels of 20-30% of breast cancers. It activates growth factor signalling pathways and is therefore associated with more aggressive cancer growth and poorer outcomes.Trastuzumab (Herceptin) is a monoclonal antibody, a drug that binds to and decreases the activity of HER2, improving outcomes for such patients. 6
1 Martin M, Kroetz D Pharmacotherapy 2013;33(7):765–775) doi: 10.1002/phar.12782 Lennard, L. (2014), “Implementation of TPMT testing”. British Journal of Clinical Pharmacology, 77: 704–714. doi: 10.1111/bcp.122263 Keeling, D., Baglin, T., Tait, C., Watson, H., Perry, D., Baglin, C., Kitchen, S., Makris, M. and British Committee for Standards in Haematology (2011), “Guidelines on oral anticoagulation with warfarin – fourth edition”. British Journal of Haematology, 154: 311–324. doi: 10.1111/j.1365-2141.2011.08753.4 Holbrook A, Schulman S, Witt DM et al. “Evidence-based management of anticoagulant therapy: antithrombotic therapy and prevention of thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines”. Chest 2012: 141 (Suppl. 2): e152S–e184S.5 Druker, Brian J. “STI571 (Gleevec™) as a paradigm for cancer therapy”. Trends in molecular medicine 8.4 (2002): S14-S18.6 Slamon, Dennis J., et al. “Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2.” New England Journal of Medicine 344.11 (2001): 783-792.
The Genomics Era: the Future of Genetics in Medicine
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