Adeniyi Bukola

Adeniyi Bukola

I am Adeniyi Bukola Joshua, a research scientist at the department of Plant Biotechnology, National Biotechnology Development Agency (NABDA) Owode Nigeria.

Location Nigeria

Achievements

Activity

  • Adeniyi Bukola made a comment
    Installation/ Setup of Linux/ a Linux-like Environment and Data Download

    I'm still unable to download this

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    Introduction to Week 1 by Martin and Mark

    Am ready and I expect to gain a wide varieties of techniques

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    Glossary

    Thanks for the descriptions

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    What the Next Three Weeks are About and Who are Your Educators?

    It's a pleasure meeting you

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    Learning Together

    Very useful information
    Thanks

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    Welcome to the Course!

    I am a plant pathologist and my research focus has been on Phage biology so bioinformatics is very key to my work. I joined this course to know more and widens my knowledge in the major key aspect of bioinformatics

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    Acknowledgements

    Thank you all

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    What is next?

    Thank you

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    Concluding discussion

    This course greatly impactful. And it has widen my knowledge in the area of AMR and Bioinformatics.
    I will directly apply the new skills have learnt in my new line of research in combating AMR using Phage therapy.

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    Summary of Week 3

    Thank you for the wonderful weeks

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    How is your learning progressing?

    The 3rd week has been a great and challenging week, the areas that deals with bioinformatics has a most challenges for me, as I need more clarity on it

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    One Health approach and the future of AMR

    Awareness and sensitization of the rural masses is very key
    I would suggest Phage Biology. A lot of research has been done on bacteriophage as a potential alternatives to combat the bacterials that has become like a super bug against antibiotics.

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    Case study 2: extensively drug-resistant typhoid outbreak in Pakistan

    This session is educative

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    Origin and global spread of the seventh Cholera pandemic

    Excellent

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    Case study 1: History of the seventh cholera pandemic in Africa

    Thank you for this

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    Introduction to Microreact

    Informative

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    Introduction to phylogenetic trees

    Informative and interesting

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    Using Artemis Comparison Tool (ACT) to browse a XDR Typhoid genome

    Am having issues with the downloading

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    Comparing the genotype and phenotype

    In a case of a mutants gene

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    Detection of resistance using Pathogenwatch

    Interesting

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    ENA database and assembly

    Thanks

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    Importance of whole genome sequencing

    Thanks

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    Welcome to Week 3

    I hope I do get the clearer picture on sequencing, AMR and Bioinformatics

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    Summary of Week 2

    Thank you for the wonderful week we had.

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    How is your learning progressing?

    This week is basically amazing and educative. Have learnt series of processes in Sequencing and the Laboratory practicals was also awesome

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    Interview with Dr Estée Török

    Thank you. This is great

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    Nanopore sequencing

    Interesting

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    Sequencing technologies

    This is well explained

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    Polymerase Chain Reaction (PCR)

    Informative

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    DNA extraction and QC

    Have undertook only PCR

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    Other techniques available

    Disc diffusion method! It's very cheap, easier and readily available.

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    Other AST methods

    Thank you

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    Broth Microdilution

    Interesting! Thank you for this

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    Limitations and quality control

    Q1
    1. The innoculum used is too light or thick.
    2. Wrong media used , check the composition of the media .
    3. Check of the antibiotics disc is expired, get a new batch if so.
    Q2: Using MIC test and genomic tools.

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    Interpretation

    Isolate 1 is resistance to all the 3 antibiotics
    Isolate 2 is susceptible to all the 3 antibiotics as evidence as this:
    Isolate 1:-
    Gentamicin(CN10) = 6mm
    Cefpodoxime(CPD10) = 6mm
    Ciprofloxacin(CIP5)- 11mm
    Isolate 2:-
    Gentamicin(CN10) = 28mm
    Cefpodoxime(CPD10) = 30mm
    Ciprofloxacin(CIP5) = 41mm

  • Adeniyi Bukola made a comment
    Reading the results

    Isolate 1:-
    Gentamicin(CN10) = 6mm
    Cefpodoxime(CPD10) = 6mm
    Ciprofloxacin(CIP5)- 11mm
    Isolate 2:-
    Gentamicin(CN10) = 28mm
    Cefpodoxime(CPD10) = 30mm
    Ciprofloxacin(CIP5) = 41mm

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    AST method

    Thanks

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    Antimicrobial susceptibility testing

    Thank you

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    Bacterial identification techniques

    Awesome

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    How do you identify bacteria?

    I have used Gram staining techniques, PCR, Motility testing, and Culture

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    Importance of correct bacterial identification

    Thanks

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    Quality assurance / Quality Control and external validation / Quality Management

    Very Crucial!

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    Aseptic technique

    Ideal!

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    Unsafe practice in the lab

    1. No gloves
    2. Open lab coat
    3. Unlabelled jar of liquid
    4. Pipette tip left on the pipette
    5. Overflowing waste jar
    6. Open waste bottle
    7. Too many wires
    8. Bacterial plate left on the bench with no cover

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    Health and Safety in the lab

    Useful and vital informations

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    The importance of good quality laboratory work

    It could be devastating if not properly managed, because it could leads to misdiagnosis.

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    Welcome to Week 2

    Excited

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    End of Week 1 Summary

    Awesome! Thank you all

  • Adeniyi Bukola made a comment
    How is your learning progressing?

    It's a wonderful experience I had for the first week, the test was quite challenging and eye opener. It's quite interesting getting to learn new things. Transposons, Plasmids, Chromosomal Mutation and recombination are very interesting topics I took much time to learn.

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    Selection

    Useful information

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    Mixed mechanisms

    Interesting!

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    Transposons

    Thank you for this

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    Plasmids

    This is agreeable

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    Chromosomal mutation and recombination

    For every bacterial there comes with a virus that infect them. Bacteriophage are viruses that interact with bacterial and this interaction brings about transfer/exchange of genetic materials.

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    Mechanisms of antimicrobial resistance

    The overview is informative

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    Intrinsic resistance

    They do intrinsically resistance for survival

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    The extent of the problem

    AMR is a huge threat now in my country especially in the area of Agriculture

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    Global perspective

    AMR is becoming a huge problem now in my country especially in the areas of Aquaculture and poultry production

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    History of antibiotic discovery and the emergence of resistance

    Thanks

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    What is the problem?

    Very informative

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    Why do we need antibiotics?

    On the real sense antibiotics are used to suppress /kill the growth of bacterial. They are more powerful in the fight against bacteria infection at all levels

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    How do antibiotics work?

    It's mainly for their survival

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    What are antibiotics?

    The continuous usage of certain antibiotics had made some certain strains of bacterial developed a couping mechanism and resistance it

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    Bacterial infections

    Most Bacterial are becoming a super bug against antibiotics.

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    Welcome to Week 1

    Looking forward to learn more

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    Meet the Educators

    It's a pleasure to meet you all

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    What the next three weeks are about

    My current line of research is focused on AMR, so all this courses under listed here are of great benefit to my career

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    Learning together

    Am so excited to be here

  • Adeniyi Bukola made a comment
    Welcome to the course

    I am Adeniyi Bukola Joshua, a scientific officer at the Department of Plant Biotechnology, National Biotechnology Development Agency (NABDA) Nigeria . I had a Masters degree in Plant Pathology from the Federal University of Agriculture Abeokuta Nigeria, I am a passionate researcher and my research focus is on Antimicrobial Resistance using Phage Biology

  • Adeniyi Bukola made a comment
    Acknowledgements

    This course came in at the time its needed most. Thanks to you all.

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    Next steps

    Thank you for the impacting courses and topics you have pool together

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    Ending remarks for the course

    I have learnt a lot from the facilitators and in the team of instructors of this course. Thank you.

  • Adeniyi Bukola replied to Anna Seale
    Ending remarks for the course

    I sincerely appreciate the organiser of this course, for putting such an educative and impacting courses in this period when its needed most.

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    Reflections on the public health response

    The Global responses most of this epidemics is quite not impressive at an initial stage. Some individuals even joking called corona viruse a Chinese virus. Most countries begin to wake to the reality of the epidemic when the severity has become more devastating and with that a bottom-up approach could not be used again.

  • Adeniyi Bukola replied to Anna Seale
    Reflections on the public health response

    Noted

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    How can vaccines help?

    There is need to step up and act as fast as possible in the production of the vaccines for this novel virus. The more the delay the more the devastating effect.

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    What more do we need to know about treatment of COVID-19?

    Most the patients that have recovered and tested negative after they had been infected, is there a chance of them taking those drugs through their entire life time. As evidence with those taking Antiretroviral drug

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    How can modelling help?

    Its gonna be great learning some essential tool in calculating the spread of disease like COVID-19, tools like Mathematical, Statistical and Economical Models.

  • Adeniyi Bukola replied to Judith Glynn
    How can modelling help?

    Thanks for sharing the links

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    How can we better understand spread?

    Its going to be an interesting 3rd week

  • Adeniyi Bukola replied to Anna Seale
    How can we better understand spread?

    The major factors are Crowded area or gathering, Contact with an infected person through body or respiratory fluids, Lack of Personal hygiene

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    Welcome to Week 3

    Thank you Dr. Mary, I look forward to learning new ideas this week

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    Reflections on the public health response

    Interesting

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    Physical distancing as a public health intervention

    We're on a lockdown here in Lagos Nigeria, as movements is been restricted. Directives had been given that most work should be done online now, now social gathering and religious activities for the period of 14days

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    Individual measures to limit transmission

    Yes I agreed to all this precautionary methods

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    Responding in Africa

    I fear my dear country Nigeria, the spread of this virus is preparing to get to the stage of community transmission which could be very devastating. As at now most big cities are been or about to lockdown which would have a negative effect of the economy and livelihoods of the people as poverty alleviating interventions are not been put to place

  • Adeniyi Bukola replied to Anna Seale
    Response in Singapore

    @AnnaSeale Yes But its not that effective

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    Response in Singapore

    contract tracing methods involving the taskforce has been employed in Nigeria but it is not effective as expected as most of the foreign countries returnees gave fake addresses to which is very difficult to trace

  • Adeniyi Bukola replied to Anna Seale
    Response in China

    Interesting response against COVID -19

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    Infection Prevention and Control in a health care setting

    Insightful

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    Psychological effects

    Yes

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    Evidence base for airport screening

    If we must curtail the spread of this virus, this General screening is inevitable

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    Quarantine - why do it?

    Self isolation is very key in reducing the virus spread,. If movement of people could be restricted transmission of the COVID -19 would also be drastically reduced

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    International UN strategic response and WHO coordination

    More interventions are still desperately needed in sub-Sahara African as Testing kit and protective materials and clothing are not adequately distributed.

  • Adeniyi Bukola replied to Anna Seale
    International UN strategic response and WHO coordination

    Noted, Thank you

  • Adeniyi Bukola made a comment
    Overview of a public health response

    All the above strategic objectives and principles are very key in mitigating the spread of this COVID-19. Most of the COVID-19 cases been experienced here now in Nigeria are from those that have recently travel or have a travelled history to those countries where the virus is pandemic. At this stage Nigeria is putting every mitigating effort to curtail...

  • Adeniyi Bukola replied to Jonathan Wong
    Welcome to Week 2

    Thank you Jonathan

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    Welcome to Week 2

    Thank you for the outline

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    What are the key points in the outbreak to date?

    Now I have the insight of the beginning of this epidemics

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    Overview of the coronavirus and COVID-19

    Looking forward to learn a new things