Visualization of resistome composition in ResistoXplorer

In this video, we will see how we can visualise and interpret resistome data in ResistoXplorer based on the real example data set: ‘Pig and Broiler’. We recommend you follow along in your own computer and try to answer some of the questions below in the exercise section.

Hands-on Exercise:

Go to the ResistoXplorer and upload your own data or use an example dataset, such as the ‘Pig and Broiler’, in ARG table module and complete the processing step with default parameters. Once you reached the Analysis Panel page, you can see the “Visual and Hierarchical” modules under Composition profiling section. Click on each one of them and visualize the resistome composition with different approaches and answer the following questions:

• What are the differences in resistome composition between the different animal hosts?
• Do you have any biological interpretation for these differences?
• Apart from the differences between the animal hosts, what are the other striking differences between samples?
• Are the differences in the resistome composition between the ‘Pig and Broiler’ samples exists at all functional levels or only at ARG level?