Performing ordination analysis of resistome data using ResistoXplorer

In this video, you will learn how to perform and infer results from beta diversity analysis in ResistoXplorer. Based on it, you need to complete the exercise present below.

Exercise:

Go to the ResistoXplorer and upload your own data or use an example dataset, such as the ‘Pig and Broiler’, in ARG table module and complete the data filtration and normalisation step. Once you reached the Analysis Panel page, you can see the “Ordination analysis” module under Composition profiling section. Click on it and visualise the differences in resistome composition between the samples or groups using different distance measures, functional levels and ordination methods. Based on that, try to answer some questions:

• Do you observe any grouping pattern in your data? If yes, then which group are more distinct from each other?
• What kind of hypothesis can you test on your data with beta diversity or ordination analysis?