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Polymerase Chain Reaction

Polymerase chain reaction PCR, is an in-vitro technique to duplicate or copy the genes or gene fragment. For example, there’s a gene that we want. And we can dip duplicate them by PCR technique. 1 into 2 and a 2 into 4. In essence, it’s an exponential amplification. And in theory, it would go for 35 cycles as long as we provide enough primer and the enzymes. So, in theory, we can duplicate billions of copies of the gene or gene fragment . And the PCR machine has become very common equipment in any biochemistry laboratory.

Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make multitude of copies of a specific DNA sample, allowing scientists to study it in detail. One very useful application is in forensic medicine, where crime scene specimen often exists in trace quantity.

Sequential narratives for the slides: When the double-stranded template DNA is heated, it is separated into two single strands (Denaturation). Then, the temperature is lowered to enable the DNA primers to attach to the template DNA (Annealing) Lastly, the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme (Extending).

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