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Polymerase Chain Reaction


Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make multitude of copies of a specific DNA sample, allowing scientists to study it in detail. One very useful application is in forensic medicine, where crime scene specimen often exists in trace quantity.

Sequential narratives for the slides: When the double-stranded template DNA is heated, it is separated into two single strands (Denaturation). Then, the temperature is lowered to enable the DNA primers to attach to the template DNA (Annealing) Lastly, the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme (Extending).

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