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Recombinant DNA and Hybridoma Technology

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So what is recombinant DNA? Let’s say we have molecule A of DNA and then a molecule B of DNA. We digest them with the same restriction endonuclease. So, in essence, we cut the structure open with the sticky ends. And if we glue them together again with DNA ligase. Now we have a new product and it is a recombinant DNA product. And by the way, endonuclease, has a Nobel Prize and the DNA ligase has a Nobel Prize as well. monoclonal antibody production. This slightly is very interesting, that’s why I would like to show. This mouse, this poor mouse, covered up with his eyes with his right hand. And the stick out his left arm for immunization.
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Now after immunization, antibody-forming cells are produced. And these cells are or were harvested and infused with tumor cell to form the so-called hybridoma. And the reason we use tumor cells is because tumor cells are quickly to grow. And we want their hybridoma cells to grow quickly as well. And the hybridoma cells were cultured and reproduced and we separate out those hybridomas that have the potential for antibody production. And we cloned them again to multiply them. And eventually, we separate out the antibody and these antibodies are derived from the very single source. And that’s they are called monoclonal antibody.

This series of slides introduces recombinant DNA technology and the hybridoma monoclonal antibody technology, which contribute to the creation of modern therapeutic treatments for cancer, autoimmune disorders and a host of other diseases.

Slides are provided on the link below. The first few slides illustrate the principles and the step by step “cut and paste” procedures for recombinant DNA technology. The remaining slides depict the hybridoma technology used in the production of monoclonal antibody with animals. Later on, recombinant monoclonal antibodies (rAbs) came to light, which are generated in vitro using synthetic genes.

If you want to know the importance of recombinant DNA, here is a reference for you :Role of Recombinant DNA Technology to Improve Life

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Pharmacotherapy: Understanding Biotechnology Products

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