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How the gut microbiota can be studied?
Here you will follow the methodology used to analyse the gut microbiota.
In this video, learners will see the different technologies nowadays available for the analysis of the microbiota microbiome from the human gut. A look into the gut can show the presence of microbes and their genes and molecules. Microbes, bacteria, yeast, moulds, and virus are able to degrade food and nutrients to produce molecules that have an impact on the host. The totality of microbes, molecules, and genes is called a “microbiome.”
The first step in the process is the DNA extraction. DNA isolation is obtained from stool samples.
In this step, you can see how to sequence DNA.
The double-stranded DNA is then broken into a single strand.
After this, the single strand of DNA is then broken into small pieces. Each single piece is then read by a machine.
From the mix of the gene sequence, we can assemble and then reconstruct the original genomes, or we can list the genes that create the code for specific protein or molecule.
Here are the possible outputs of the sequencing– microbiota composition, genome reconstruction, drawing metabolic pathways that can be used to produce molecules.
Are you curios to know how the gut microbiota/microbiome can be analysed? In this video we will show you the technologies nowadays available to determine this, starting from the type of samples utilized, to the sequencing and the presentation of the results.
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