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Viewing your first DNA sequence in Artemis

Opening a FASTA file in Artemis and showing some of the basic functionality, including the navigation tools.

In this video we will demonstrate how to open a sequence FASTA file in Artemis. We will explore the look of Artemis and some basic tools for navigation.

Before you start with the video, take a moment to download the Salmonella typhi genome sequence in FASTA format from the Wellcome Sanger Institute FTP site. We recommend use of Chrome or Firefox browsers for downloading data files.

ftp://ftp.sanger.ac.uk/pub/resources/coursesandconferences/Online_Courses/Course3/data/S_typhi.dna

You may need to copy and paste the link in your internet browser. This will open the genome sequence itself in your internet browser. To save it to a file, choose the Save As option from your genome browser, and save the file with the name St.dna.

When the video is playing, feel free to follow along on your computer.

Note: Depending on your computer, you may not be able to choose to Open the St.dna file right away from Artemis, as demonstrated in the video. In some cases, you may be required to switch the drop-down menu option of “File type” to ““All file types”. Then, the sequence file St.dna will become available.

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Bacterial Genomes II: Accessing and Analysing Microbial Genome Data Using Artemis

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