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How to isolate RNA from the sample?

High-quality RNA is key for molecular techniques like RT-qPCR and sequencing. Steps include sample collection, RNA preparation, and quantitation.

Before RNA extraction, we have to derive high-quality RNA from samples.

Which means the sample needs to be protected completely and collect it in the right way. High-quality RNA can be used in performing many molecular techniques such as reverse transcription real-time PCR (RT-qPCR), transcriptome analysis using next-generation sequencing, array analysis, digital PCR, northern analysis, and cDNA library construction.

Step one is to collect and preserve samples. RNAlater solution allows the researcher to postpone RNA purification even for months after tissue collection.

Step 2: There are two RNA preparation technologies that are widely available: organic extraction methods and spin basket formats.

Step 3: Using A260/A280 ratio quantitate isolated RNA to make sure it’s highly purified RNA. Last but not least, it’s crucial to check the quantity and quality of RNA by Nanodrop One Spectrophotometers.

Review question: In order to derive high-quality RNA from the samples, what’s the process to extract RNA and how to quantitate isolated RNA?

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