Absolute vs. Relative Quantification for qPCR
Please take some time to read the following website about absolute and relative quantification for qPCR . When we are calculating the results of real-time PCR experiment, there are two …
Course: Molecular Biology: How to Detect Pathogens in Aquaculture
How can we select housekeeping gene(s) for real-time PCR data analysis?
How can we select housekeeping gene(s) for real-time PCR data analysis? Selecting a (or more than one) proper housekeeping gene(s) is not an easy job. RT-qPCR is frequently chosen as …
Rapid Detection of Enterocytozoon hepatopenaei Infection in Shrimp With a Real-Time Isothermal Recombinase Polymerase Amplification Assay
Please take some time to read the following article: Rapid Detection of Enterocytozoon hepatopenaei Infection in Shrimp With a Real-Time Isothermal Recombinase Polymerase Amplification Assay . Hepatopancreas microsporidian was first …
Immunological-based assays for specific detection of shrimp viruses
Please take some time to read the following article: Immunological-based assays for specific detection of shrimp viruses In this article, several shrimp viral agents will be introduced. And in addition …
What is quantitative real-time PCR (qPCR)? Ct value and PCR efficiency
Real-time PCR is the ability to monitor the progress of the PCR as it occurs. Data is collected throughout the PCR process to quantitate DNA and RNA. By quantitation assays, …
Viral shrimp diseases listed by the OIE: a review
Please read this research regarding shrimp disease. Viral Shrimp Diseases Listed by the OIE: A Review The article examines the various viral diseases that affect shrimp and have been designated …
How does PCR work?
In the PCR process, the target DNA will amplify samples double times each run, therefore, you will get 109 copies after 30 runs. Within a run, the temperature inside the …
Perform cDNA synthesis and cDNA amplification in PCR
Step5: cDNA synthesis performing. Controlling changes of temperature makes all components react properly. From primer annealing to DNA polymerization, then, enzyme deactivation, you would get cDNA after reacting. Therefore, using …
Preparation of PCR
In the beginning, we should check the list on PCR preparation to make sure all equipment and components are ready before the PCR process. For instance, DNA template, primers, DNA …
Step to optimal cDNA synthesis part 1: Sample preparation
The synthesis of DNA from an RNA template, via reverse transcription, results in complementary DNA (cDNA). There are five steps to synthesize cDNA. Step 1: Sample preparation. Step 2: Remove …
Step to optimal cDNA synthesis part 2: Primer selection and reaction mix
Before going to step 4, primer selection is very important for your target RNA. There are three types of primers usually used in reaction: Oligo(dT) primers, random primers, and gene-specific …
Shrimp viral diseases, import risk assessment and international trade
Please take some time to read this article, Shrimp Viral Diseases, Import Risk Assessment, and International Trade. Shrimp is a significant commodity in the international market, accounting for 15% of …
How to isolate RNA from the sample?
Before RNA extraction, we have to derive high-quality RNA from samples. Which means the sample needs to be protected completely and collect it in the right way. High-quality RNA can …
Prevention of 3 common disease in shrimp
Please take some time to read this article, Prevention of White Feces Syndrome, White Gut Disease and White Muscle Disease in Shrimp. The website provides information on the prevention of …
Methods for identifying aquaculture diseases
Aquaculture, particularly intensive or super-intensive high-density breeding, can lead to disease outbreaks in fish and shrimp due to microbial infection. One notorious disease is acute hepatopancreatic necrosis disease (AHPND) or …
These articles are from the online course:
Molecular Biology: How to Detect Pathogens in Aquaculture