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Absolute vs. Relative Quantification for qPCR

Please take some time to read the following website about absolute and relative quantification for qPCR . When we are calculating the results of real-time PCR experiment, there are two …

How does PCR work?

In the PCR process, the target DNA will amplify samples double times each run, therefore, you will get 109 copies after 30 runs. Within a run, the temperature inside the …

Perform cDNA synthesis and cDNA amplification in PCR

Step5: cDNA synthesis performing. Controlling changes of temperature makes all components react properly. From primer annealing to DNA polymerization, then, enzyme deactivation, you would get cDNA after reacting. Therefore, using …

Preparation of PCR

In the beginning, we should check the list on PCR preparation to make sure all equipment and components are ready before the PCR process. For instance, DNA template, primers, DNA …

How to isolate RNA from the sample?

Before RNA extraction, we have to derive high-quality RNA from samples. Which means the sample needs to be protected completely and collect it in the right way. High-quality RNA can …

Prevention of 3 common disease in shrimp

Please take some time to read this article, Prevention of White Feces Syndrome, White Gut Disease and White Muscle Disease in Shrimp. The website provides information on the prevention of …

PCR procedures

PCR is a fast and inexpensive technique used to “amplify“, or copy, small segments of DNA. Because significant amounts of a sample of DNA are necessary for molecular and genetic …