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Step to optimal cDNA synthesis part 1: Sample preparation
The synthesis of DNA from an RNA template, via reverse transcription, results in complementary DNA (cDNA). There are five steps to synthesize cDNA.
The synthesis of DNA from an RNA template, via reverse transcription, results in complementary DNA (cDNA). There are five steps to synthesize cDNA. Step 1: Sample preparation. Step 2: Remove genomic DNA. Step 3: Reverse transcriptase selection
Review question: What’s the most efficient way to remove gDNA? How to choose the right reverse transcriptase to reduce the reaction time?
This article is from the online course:
Molecular Biology: How to Detect Pathogens in Aquaculture
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Molecular Biology: How to Detect Pathogens in Aquaculture
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