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Step to optimal cDNA synthesis part 2: Primer selection and reaction mix

There are three types of primers usually used in reaction: Oligo(dT) primers, random primers, and gene-specific primers.

Before going to step 4, primer selection is very important for your target RNA. There are three types of primers usually used in reaction: Oligo(dT) primers, random primers, and gene-specific primers. Make sure that the primers are suitable for your experiment design. Step 4: Reaction mix preparation. In addition to enzymes and primers, the main reaction components for reverse transcription include RNA template, buffer, dNTPs and RNase inhibitor etc.

Review question: What’s the appropriate primers for cDNA synthesis in the video? what’s the key benefits of it? What’s the list of reaction mix preparation?

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Molecular Biology: How to Detect Pathogens in Aquaculture

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