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Perform cDNA synthesis and cDNA amplification in PCR

Controlled temperature changes ensure proper cDNA synthesis. PCR amplification enables sufficient cDNA for detection.

Step5: cDNA synthesis performing. Controlling changes of temperature makes all components react properly. From primer annealing to DNA polymerization, then, enzyme deactivation, you would get cDNA after reacting. Therefore, using PCR to amplify cDNA makes you have enough quantity of cDNA to detect and go on to the next steps.

Review question: What’s the reason to use a thermostable reverse transcriptase? what’s the temperature changes in performing cDNA synthesis and cDNA PCR?

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Molecular Biology: How to Detect Pathogens in Aquaculture

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