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Preparation of PCR

Before PCR, ensure all equipment and components are ready, such as DNA template, primers, polymerase, buffer, and machine.

In the beginning, we should check the list on PCR preparation to make sure all equipment and components are ready before the PCR process. For instance, DNA template, primers, DNA polymerase, buffer and PCR machine are necessary.

Review question: What are the components on your PCR preparation list? How much volume is required?

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Molecular Biology: How to Detect Pathogens in Aquaculture

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