Skip main navigation

Using Carbon 14 to analyse human skeletal remains

The Carbon 14, or radiocarbon dating method is one of the best-known methods of dating human fossils and has been around since the late 1940s.

The Carbon 14, or radiocarbon dating method is one of the best-known methods of dating human fossils and has been around since the late 1940s.

The Carbon 14 (14C) dating method is a radiometric dating method. A radiometric dating uses the known rate of decay of radioactive isotopes to date an object. Each radioactive isotope has a known, fixed rate of decay, which we call a half-life. The half-life is the amount of time it takes for a quantity to fall to half of the value that it started with.

This means that if we know the isotope and its rate of decay, then we can calculate how old the substance is.

So now we need a little science lesson about carbon.

The element carbon occurs in nature in three isotopic forms. Carbon 12 (12C) is stable and represents 98.9% of the carbon in the atmosphere. The rest (1.1%) is mostly made up of Carbon 13 (13C), which is also stable, and Carbon 14 (14C) which is unstable – or radioactive. In our atmosphere, only about one in a trillion carbon atoms is 14C.

Most of the 14C in our atmosphere is produced in the upper atmosphere by the action of cosmic rays on nitrogen (14N) to produce 14C. Once 14C is produced, it starts to decay back to nitrogen. The atmosphere has constant levels of 14C – the production of new 14C in the atmosphere and the decay of 14C balance each other in a steady state equilibrium.

These three different forms of carbon are oxidised and dispersed through our atmosphere. The oxidised carbon is absorbed by plants through photosynthesis. Once it is in the plants, it enters the food chain. When the 14C enters the plant or animal, it remains in equilibrium with the atmosphere. However – once the tissue dies (i.e. the animal dies or a tree’s sapwood converts into hardwood) then the tissue is no longer being replaced and the level of 14C continuously decreases through radioactive decay.

Cosmic rays interact with particles in the atmosphere, producing thermal energy and isotopes of nitrogen and carbon. These isotopes then enter their respective cycles and become invloved with the photosynthsis of plants and oxidation.

14C entering the food chain (Image: © Fiona Grün)

If we know how much 14C was in the living tissue, we can measure the amount of 14C in the dead plant or animal and then compare these to assess how long it has been dead. We can do this because we know the decay rate of 14C (it has a half-life of 5,730 years). The result is the radiocarbon age of the sample.

But how do we know?

The next question, of course, is – how do we know the amount of 14C that was in the living tissue when it died? Well, this isn’t simple to determine. As it turns out, the production of 14C in the atmosphere has not been constant over time, because of fluctuations in the amount of cosmic rays reaching the earth. We’ve been able to confirm this by measuring tree rings and comparing their known age with the radiocarbon result. This allowed us to create carbon calibration curves. However, our tree ring chronology only goes back 12,000 years (look up dendrochronology if you are especially interested in this aspect of dating). So, we’ve had to use other materials such as corals, speleothems, lake sediments and ice-cores to create calibration curves for radiocarbon dating. We are working hard to extend these calibration curves as much as possible – currently we are at around 55,000 years.

This means that radiocarbon dates are given as a calibrated date – which is our best estimate based upon our current calibration curves, but also introduces a margin for error. As research continues to update our calibration curves, we need to go back to the original radiocarbon dates and recalibrate them.

That’s one challenge.

Remember that we said earlier that the naturally occurring amount of 14C in the atmosphere is about one in one trillion carbon atoms? That’s another challenge. In such small amounts, 14C is very difficult to measure and it is also very sensitive to contamination. When we first started using radiocarbon dating, huge samples were required. Today we use (very expensive) accelerator mass spectrometers (AMS) to count 14C atoms in a sample. And we are also constantly researching new methods of sample preparation to help reduce the likelihood of contamination.

When using radiocarbon dating for more recent dating, we also need to take into consideration recent human activities that have changed the global levels of 14C. These are the burning of fossils fuels (called the “Suess effect”) and testing of nuclear weapons (called the “bomb effect”). The Suess effect has lowered the radiocarbon concentration in the atmosphere. The Bomb effect has dramatically increased it. Studies have put this increase as high as 100% by 1963 (Baxter and Walton 1970). These effects mean that radio carbon dates need to be calculated to a “pre-bomb” age of 1950. Tested material that has died since 1950 have levels of radiocarbon that is so high that their ages are reported as a “percent modern (1950)”.

All of this combines to produce dates that can be difficult to assess as correct. Certainly, a single radiocarbon dating of a specimen should be treated with caution.

In the laboratory video we show you how we extract bone collagen from a sample to give us original, unaltered bone material to test in the AMS. You’ll see that it’s quite a process.

Your task

What did you think of the process shown in our video?

Do you think radiocarbon dating is a valid method of dating human remains?

Select the comments link and share your thoughts


Aitken, M.J. (1990). Science-based dating in Archaeology. Longman Inc., New York.

Baxter, M.S. and Walton, A., (1970). A theoretical approach to the Suess effect, Proceedings of the Royal Society of London. Series A, Mathematical and Physical Sciences. 318, 1533 (Aug. 11), 213-230.

Grün, R. (2006). “Direct Dating of Human Fossils”. Yearbook of Physical Anthropology, 49: 2-48

Wood, R. (2012). Explainer: what is radiocarbon dating and how does it work? The Conversation.

This article is from the free online

Written in Bone: An Introduction to Forensic and Bio-archaeology

Created by
FutureLearn - Learning For Life

Reach your personal and professional goals

Unlock access to hundreds of expert online courses and degrees from top universities and educators to gain accredited qualifications and professional CV-building certificates.

Join over 18 million learners to launch, switch or build upon your career, all at your own pace, across a wide range of topic areas.

Start Learning now