Colour thresholding
Often, the background and foreground in an image might be similar in brightness, but have distinct colours, or either the foreground or background might be a uniform distinct colour.
In this case, colour thresholding might perform better than thresholding on the grayscale value alone. This video shows how that is done using Fiji by using the HSV colour space, and also how once selected Fiji can be used to count distinct objects.
To try this out yourself, have a look at the practical later in the activity.
Introduction to Image Analysis for Plant Phenotyping
Introduction to Image Analysis for Plant Phenotyping
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