Skip main navigation

Exploring pseudogenes in Mycobacterium leprae

In this step you explore 5 different pseudogenes across the _Mycobacterium leprae_ genome, you will investigate their annotation and make comments on
Three Armadilloes in captivity - they are natural hosts of Mycobacterium leprae
© Wellcome Genome Campus Advanced Courses and Scientific Conferences

In this Step you will explore 5 different pseudogenes across the Mycobacterium leprae genome, you will investigate their annotation and make comments on their GC content.

Previously we described just one pseudogene out of a possible 1,000 pseudogenes in M. leprae. Thankfully this is a well-annotated genome and most pseudogenes should be annotated as such.

Your first task is to scroll along the genome and select five pseudogenes. Confirm that they are indeed pseudogenes by checking their annotation and also visually inspecting that they have at least one premature stop codon.

Once you have identified your pseudogenes:

  • Write down the locus_id and any other useful information provided, such as an ortholog gene in another species and its putative function.

For each pseudogene find the GC content (or GC%):

  • Use the View menu, select “Feature Statistics”

  • You will then need to use the sequence in a BLAST (Hint: use blastx search) search to confirm the predicted functional annotation and also to confirm that the same has not been updated or changed since the genome was published.

  • Lastly, check the functional annotation in the Mycobacterium tuberculosis genome file and note down the function of its ortholog gene, if indicated in the M. leprae annotation.

Keep this information because you will need it for the next Step.

© Wellcome Genome Campus Advanced Courses and Scientific Conferences
This article is from the free online

Bacterial Genomes II: Accessing and Analysing Microbial Genome Data Using Artemis

Created by
FutureLearn - Learning For Life

Our purpose is to transform access to education.

We offer a diverse selection of courses from leading universities and cultural institutions from around the world. These are delivered one step at a time, and are accessible on mobile, tablet and desktop, so you can fit learning around your life.

We believe learning should be an enjoyable, social experience, so our courses offer the opportunity to discuss what you’re learning with others as you go, helping you make fresh discoveries and form new ideas.
You can unlock new opportunities with unlimited access to hundreds of online short courses for a year by subscribing to our Unlimited package. Build your knowledge with top universities and organisations.

Learn more about how FutureLearn is transforming access to education