Skip main navigation

Does soybean and fish oil affect inflammatory reaction?

A group of researchers investigated the impact of different ratios of soybean and fish oil mixtures upon inflammatory reactions in mice.

In some recent research, a group of researchers in Taiwan investigated the impact of different ratios of soybean and fish oil mixtures upon inflammatory reactions in mice with dextran sulphate sodium-induced acute colitis.

Team: Cyoung-Huei Huang, Yu-Chen Hou, Chiu-Li Yeh, Sung-Ling Yeh

Background & aims

Inflammatory bowel disease is a recurrent disease of the gastrointestinal tract. n-3 polyunsaturated fatty acids (PUFAs) are proved to have anti-inflammatory and immunomodulatory properties. This study evaluated the effects of different dietary n-6/n-3 PUFA ratios on the mechanism of alleviating the inflammatory response in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods

Mice were randomly assigned to 6 groups including 3 non-colitis groups (C, LF, and HF) and 3 colitis groups (DC, DLF, and DHF). Mice in the C and DC groups were fed a common semipurified diet with soybean oil as the fat source. The other groups received an identical component except that part of the soybean oil was replaced by different amounts of fish oil. The n-6/n-3 PUFA ratio of the LF and DLF groups was 4:1, the ratio of the HF and DHF groups was 2:1. After feeding the respective diets for 2 weeks, the colitis groups were given distilled water containing 2% DSS, while the non-colitis groups were given distilled water for 5 days. After that, all mice were sacrificed at the recovery phase after drinking distilled water for another 5 days.

Results

Colitis resulted in higher expressions of colonic inflammatory mediators in colon tissues and colon lavage fluid. Also, colonic peroxisome proliferator-activated receptor (PPAR)-g and the IkBa/nuclear factor (NF)-kB p65 ratio were lower than those of the non-colitis groups. Compared to the DC group, fish oil-enriched colitis groups had lower inflammatory mediator expressions and higher PPAR-g protein levels and IkBa/NF-kB p65 ratios in colon tissues. The DHF group had even lower colonic inflammatory gene and higher PPAR-g protein expressions than did the DLF group. Conclusions: These findings suggest that diets enriched with fish oil upregulated PPAR-g and decreased NF-kB activation that may consequently have reduced luminal inflammatory mediator production. Compared to a n-6/n-3 PUFA ratio 4:1, a ratio of 2:1 was more effective in reducing inflammatory re- actions in DSS-induced colitis.

This article is from the free online

Nutrition and Disease Prevention

Created by
FutureLearn - Learning For Life

Our purpose is to transform access to education.

We offer a diverse selection of courses from leading universities and cultural institutions from around the world. These are delivered one step at a time, and are accessible on mobile, tablet and desktop, so you can fit learning around your life.

We believe learning should be an enjoyable, social experience, so our courses offer the opportunity to discuss what you’re learning with others as you go, helping you make fresh discoveries and form new ideas.
You can unlock new opportunities with unlimited access to hundreds of online short courses for a year by subscribing to our Unlimited package. Build your knowledge with top universities and organisations.

Learn more about how FutureLearn is transforming access to education